Recent publication

 Nakai, H.; Isshiki, K.; Hattori, M.; Maehira, H.; Yamaguchi, T.; Masuda, K.; Shimizu, Y.; Watanabe, T.; Hohsaka, T.; Shihoya, W. et al.(2022) "Cell-Free Synthesis of Human Endothelin Receptors and Its Application to Ribosome Display."  Anal Chem, 94 (9), 3831.

Ghanem, N.; Kanagami, N.; Matsui, T.; Takeda, K.; Kaneko, J.; Shiraishi, Y.; Choe, C. A.; Uchikubo-Kamo, T.; Shirouzu, M.; Hashimoto, T. et al. (2022) "Chimeric mutants of staphylococcal hemolysin, which act as both one-component and two-component hemolysin, created by grafting the stem domain." FEBS J.DOI:10.1111/febs.16354 10.1111/febs.16354.

Uyeda, A.; Reyes, S. G.; Kanamori, T.; Matsuura, T. (2022) "Identification of conditions for efficient cell-sized liposome preparation using commercially available reconstituted in vitro transcription-translation system.J Biosci Bioeng, 133 (2), 181. 

You can receive the latest publication news also from twitter account @BottomUBiotech

Selected publication list

  1. Xu, C., Lu, P., Gamal El-Din, T. M., Pei, X. Y., Johnson, M. C., Uyeda, A., Bick, M. J., Xu, Q., Jiang, D., Bai, H., Reggiano, G., Hsia, Y., Brunette, T. J., Dou, J., Ma, D., Lynch, E. M., Boyken, S. E., Huang, P. S., Stewart, L., DiMaio, F., Kollman, J. M., Luisi, B. F., Matsuura, T., Catterall, W. A., and Baker, D. (2020) Computational design of transmembrane pores. Nature 585, 129-134 link
  2. Noba, K., Ishikawa, M., Uyeda, A., Watanabe, T., Hohsaka, T., Yoshimoto, S., Matsuura, T., and Hori, K. (2019) Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA. J Am Chem Soc 141, 19058-19066
  3. Dwidar, M., Seike, Y., Kobori, S., Whitaker, C., Matsuura, T., and Yokobayashi, Y. (2019) Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch. J Am Chem Soc 141, 11103-11114
  4. Matsuura, T., Tanimura, N., Hosoda, K., Yomo, T., and Shimizu, Y. (2017) Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling. Proc Natl Acad Sci U S A 114, E1336-E1344
  5. Soga, H., Fujii, S., Yomo, T., Kato, Y., Watanabe, H., and Matsuura, T. (2014) In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume. ACS Synth Biol 3, 372-379
  6. Kazuta, Y., Matsuura, T., Ichihashi, N., and Yomo, T. (2014) Synthesis of milligram quantities of proteins using a reconstituted in vitro protein synthesis system. J Biosci Bioeng 118, 554-557
  7. Fujii, S., Matsuura, T., Sunami, T., Nishikawa, T., Kazuta, Y., and Yomo, T. (2014) Liposome display for in vitro selection and evolution of membrane proteins. Nat Protoc 9, 1578-1591
  8. Fujii, S., Matsuura, T., Sunami, T., Kazuta, Y., and Yomo, T. (2013) In vitro evolution of alpha-hemolysin using a liposome display. Proc Natl Acad Sci U S A 110, 16796-16801
  9. Matsuura, T., Kazuta, Y., Aita, T., Adachi, J., and Yomo, T. (2009) Quantifying epistatic interactions among the components constituting the protein translation system. Mol Syst Biol 5, 297
  10. *Kazuta, Y., *Adachi, J., *Matsuura, T., Ono, N., Mori, H., and Yomo, T. (2008) Comprehensive analysis of the effects of Escherichia coli ORFs on protein translation reaction. Mol Cell Proteomics 7, 1530-1540 (*contributed equally)
  11. Matsuura, T., Yamaguchi, M., Ko-Mitamura, E. P., Shima, Y., Urabe, I., and Yomo, T. (2002) Importance of compartment formation for a self-encoding system. Proc Natl Acad Sci U S A 99, 7514-7517
  12. Matsuura, T., Miyai, K., Trakulnaleamsai, S., Yomo, T., Shima, Y., Miki, S., Yamamoto, K., and Urabe, I. (1999) Evolutionary molecular engineering by random elongation mutagenesis. Nat Biotechnol 17, 58-61

Publication list


  • 2009年度 酵素工学研究会奨励賞「無細胞翻訳系を利用したタンパク質の機能改変に関する研究」
  • 2014年度 第3回 大阪大学総長奨励賞(研究部門)
  • 2015年度 第4回 大阪大学総長奨励賞(研究部門)
  • 2016年度 第52回 日本生物工学会研究奨励賞(斎藤賞)「セルフリータンパク質合成系を用いた進化分子工学技術の開発」
page top